Fig. 7.
p21-activated kinase 1 (PAK1) phosphorylation and vimentin phosphorylation upon stimulation with 5-HT are diminished in cells producing p47phox shRNA. Untransfected cells and cells expressing shRNAs were stimulated with 5-HT or left unstimulated. Phosphorylation of PAK1 and vimentin was determined by immunoblot analysis. Protein phosphorylation in response to 5-HT stimulation in cells producing shRNAs are normalized to the corresponding levels in unstimulated and untransfected cells. A: representative immunoblots illustrating reduced PAK1 phosphorylation at Thr-423 in cells producing p47phox shRNA upon 5-HT stimulation. B: *Significantly lower level of PAK1 phosphorylation stimulated by 5-HT in cells producing p47phox shRNA compared with untransfected cells and cells producing luciferase shRNA (P < 0.05, n = 4). C: immunoblots showing lower vimentin phosphorylation in response to 5-HT stimulation in cells producing p47phox shRNA. D: *significantly lower vimentin phosphorylation during 5-HT stimulation in cells expressing p47phox shRNA than in untransfected cells and in cells expressing luciferase shRNA (P < 0.05). Values are means ± SE (n = 4).