Characterization of mTOR transgenic mice. A: TA muscles from 10-wk-old wild-type (WT), RR, and RR/KI mice were isolated, cryosectioned, and stained with H&E. The average CSAs with SD are shown below the images (n = 4–8 mice). Scale bar represents 50 μm. B: limb muscle homogenates were isolated from mice injected with Rap (1 mg/kg) or vehicle and subjected to Western analysis for phospho-S6, a read-out for mTORC1 signaling activity. Anti-tubulin blot served as a loading control.