Fig. 5.

RR-mTOR supports RR muscle regeneration. RR-mTOR mice were subjected to TA muscle injury, Rap injection, and muscle isolation as described in Fig. 1. A: TA muscle cross-sections generated on various days AI were stained with H&E. Scale bar represents 50 μm. B: number of regenerating myofibers was counted within an area of 614,400 μm² for each sample. C: CSAs of regenerating myofibers were quantified. At least 100 regenerating myofibers were measured for each muscle section. For all the quantification data, the average results (n = 7 mice for each data point) are shown with error bars representing SD. The WT data are from Fig. 1, B and C. In B, paired two-tailed t-tests were performed to compare RR to WT samples under the same conditions (same day and same treatment), *P < 0.05. In C, two-way repeated measures ANOVA was performed to analyze regeneration in the presence of rapamycin over the time course, and between WT and RR genotypes. Overall analysis: time, P < 0.0001; genotype, P < 0.0001; interaction, P < 0.0001. Significant difference between WT and RR at each time point: **P < 0.001.