. 2009 Dec 21;4(12):e8360. doi: 10.1371/journal.pone.0008360

Table 4. Fourteen isolates and six ulcer specimens from tularemia patients in Sweden 2008 characterized by the developed hierarchical real-time PCR array.

Category	FSC no.^a	Sample ID^b	Location of the receiving hospital	Table 5 genotype	Figure 3 subclade
Isolates	792	32–92	Säffle	13	B4.Ftind49/18
	844	32–280	Uddevalla	13	B4.Ftind49/18
	780	32–51	Luleå	16	B3.23/[24], [25]
	785	32–75	Falun	16	B3.23/[24], [25]
	812	32–123	Sunderbyn	16	B3.23/[24], [25]
	816	32–142	Boden	16	B3.23/[24], [25]
	823	32–155	Lövånger	16	B3.23/[24], [25]
	831	32–173	Skellefteå	16	B3.23/[24], [25]
	794	24–95	Östersund	19	B1.20/21
	777	32–38^c	Örebro	19	B1.21/22
	787	32–79	Umeå	20^d	B1.21/22
	778	32–47^c	Ljusdal	20^d	B1.21/22
	783	32–69	Färila	21	B1.FSC200
	817	32–145	Bollnäs	21	B1.FSC200
Ulcer specimens	–	32–151^e	Jönköping^f	16, 17 or 18^g	B3.23/[24], [25]
	–	32–300^e	Gävle	16, 17 or 18^g	B3.23/[24], [25]
	–	32–87^e	Umeå	16, 17 or 18^g	B3.23/[24], [25]
	–	32–215 ^e	Uddevalla	19^h	B1.20/21
	–	32–38^c	Örebro	20^h	B1.21/22
	–	32–47^c	Ljusdal	20^h	B1.21/22

Strain ID in the Francisella Strain Collection, Swedish Defense Research Agency, Umeå, Sweden.

Sample ID at the Department of Clinical Bacteriology, Umeå University, Umeå, Sweden.

Isolate FSC777 and ulcer specimens 32–38 are from the same patient. Isolate FSC778 and ulcer specimens 32–47 are from the same patient.

The exact genotype could not be determined due to detection failure of marker B.22 (the difference in time of appearance between the two PCR products was less than one cycle).

F. tularensis cultures were negative.

The patient reported probable acquisition of tularemia when visiting the county of Jämtland, where the regional center is Östersund.

The genotype and subclade were assigned based on marker B.20, which exhibited an A for all three specimens, and on marker B.23, which exhibited a T. No other markers were screened due to scarcity of DNA.

The genotype and subclade were assigned based on: marker B.20, which exhibited a G for all three specimens; on marker B.21, which exhibited a G for specimens 32–215, and an A for specimens 32–38 and 32–47; and on marker B.22, which exhibited a G for specimens 32–38 and 32–47. No other markers were screened due to scarcity of DNA.