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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Dev Biol. 2009 Feb 4;328(2):328–341. doi: 10.1016/j.ydbio.2009.01.037

Figure 9.

Figure 9

Correlation of Pax2-Cre Dicer KO inner ear morphogenesis with developmental gene expression and residual mature miRNA expression. A, Progressive loss of Fgf10 expression in KO inner ear. At E11.5, the KO inner ear exhibits diminished but discrete expression of Fgf10 in sensory epithelia (PC, posterior crista; AC, anterior crista; HC, horizontal crista; U, utricle; CO, cochlea). At E12.5, Fgf10 expression is observed only in the posterior crista and utricular macula, and expression is entirely lost in vestibular ganglia (VG) and the developing cochlea. By E14.5, Fgf10 expression remains only in the posterior crista with faint expression in the utricular macula, in sharp contrast to extensive expression in all sensory epithelia and delaminating neurons of control littermate inner ear. B, ISH detecting miR-183 and miR-183* in KO and control littermate inner ear. Throughout the control inner ear, miR-183 expression is apparent in statoacoustic ganglia (SAG) and hair cells of developing sensory epithelia, whereas mir-183* does not accumulate as detectible pre-miRNA or mature miRNA. In the KO inner ear, miR-183 is detected primarily in the posterior crista (PC; filled arrowhead) whereas miR-183* is not (open arrowhead), thus suggesting that miR-183 detection results from residual mature miRNA production rather than detection of accumulated pre-miRNA. Labels are as indicated in the legend to Fig. 2.