Fig. 7.
LOH analysis of chromosome 15 STR markers D15S-126 and D15S-209 flanking the β 2 m gene on the RCC52 cell line, its epithelioid E3D4 and E4F5 sublines and its fibroblast-like M2C5 and M3B8 sublines. Genomic DNAs prepared from RCC52 patient’s tumor specimen and peripheral blood lymphocytes (RCC52 PBL) were analyzed in parallel with those extracted from the four RCC52 clonal sublines. Two white arrows indicate two separate alleles, i.e. retention of heterozygosity (ROH), while a single arrow indicates one allele, i.e. LOH. M: DNA size ladder in base pairs (bp)