Fig. 3.

Hyperoxia decreases expression of VEGFR-2 and eNOS but not VEGF protein in preterm ECFCs. Western blot analyses of cell lysates exposed to hyperoxia (50% oxygen) on day 6 of growth assay (HO, open bars) are compared with room air controls (RA, filled bars). A: VEGF protein in preterm ECFCs is unchanged by hyperoxia exposure. VEGFR-2 (B) and endothelial nitric oxide synthase (eNOS; C) protein in preterm ECFCs are markedly reduced in hyperoxia compared with room air controls. Error bars represent SE. *P < 0.05. n = 4.