Fig. 6.

dR cells exhibit augmented migratory capabilities compared with dS-SMC. A: migratory capabilities of dR cells and dS-SMC were compared using the “scrape” assay (see methods and Supplemental material) under both normoxic (21% O₂) and hypoxic (3% O₂, shown) conditions. Hydroxyurea (5 μM) was added to inhibit (and thus exclude contribution of) cell replication. B: statistical analysis demonstrates that 48 h after initiation of the scrape, the number of dR cells that migrated into the scrape area under normoxic conditions was 6-fold higher than that of the dS-SMC. Hypoxia (3% O₂) further augmented the migratory capabilities of dR-cells (up to 9-fold compared with dS-SMC). Shown is a representative graph from 4 independent experiments (in which cells from 3 different control and 4 hypoxic calves were used). Samples for each data point were run in triplicate. **P < 0.01; ***P < 0.01 compared with dS-SMC.