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Fluorographic detection after SDS/PAGE of photolabeled tryptic fragments of the membrane-bound protoporphyrinogen oxidase fractionated by ultracentrifugation after the trypsin digestion. Lanes: 1 and 6, molecular mass markers; 2 and 4, total labeling in the membrane fraction (2) and supernatant (4); 3 and 5, nonspecific labeling (labeling in the presence of 10 μM cold acifluorfen) in the membrane fraction (3) and supernatant (5).
