Fig. 1.

Effects of iloprost (ILO) on ventilator-induced acute lung injury (VILI). Mice were subjected to high tidal volume (HTV; 30 ml/kg, 4 h). Spontaneously ventilated animals served as controls (Con). ILO (2 μg/kg iv) was given at 0, 40, and 80 min during mechanical ventilation. Sterile saline was given as a control. A: cell count and protein concentration were measured in bronchoalveolar lavage (BAL) samples from Con and experimental animals. Results are representative of 5 independent experiments; 2–4 animals per condition. Values are means ± SD. *P < 0.01. B: lung specimens were obtained from Con, HTV, ILO, and HTV + ILO groups. Lungs were fixed in 4% parafomaldehyde, embedded in paraffin, cut into 4-μm sections, and stained with hematoxylin and eosin. Images are representative of 6–9 lung specimens for each condition. Veh, vehicle.