Figure 2.

Functional analysis of internal deletion mutants of Ste50p. (A) Schematic representation of internal deletion mutants of Ste50p. Dashed lines indicate deletions. The locations of β-strands and α-helices are indicated by arrows and coils. The dotted arrows mark the predicted but unobserved first two β-strands. (B) Yeast cells (ste50Δ ssk2Δ ssk22Δ) bearing different Ste50p mutants were assayed for their ability to grow on hyperosmolarity media. (C) Yeast cells (ste50Δ FUS1-LacZ) bearing different Ste50p mutants were assayed for their ability to activate the pheromone response pathway using a β-galactosidase reporter assay.