Table 1.
Ste50p mutants function differentially in HOG and pheromone response pathways
| STE50 alleles |
OPY2 alleles |
|||
|---|---|---|---|---|
| HOG pathway activity |
Pheromoneresponse | |||
| ΔFID (SID) | ΔSID (FID) | WT | WT | |
| Vector | − | − | − | + |
| WT | + | + | + | +++ |
| S259A | + | + | + | ND |
| R274A | − | + | + | +++ |
| H275A/N276A | + | − | + | +++ |
| N270A | + | + | + | ND |
| D281A | + | + | + | ND |
| R283A | − | + | + | +++ |
| V305T | + | + | + | ND |
| K309A | − | + | + | +++ |
| I320K | − | − | − | + |
| R274A/H275A/N276A | ND | ND | − | +++ |
Note that ste50Δ causes a severe defect in pheromone response, but is not completely sterile. Wild-type Ste50p is required for normal pheromone response (+++) as measured by halo assay and transcriptional reporter assay (see Supplemental Figure S4 for details). In the assay for HOG pathway activity, the ability to grow on hyperosmolarity media is indicated by positive sign (+) and inability to do so by negative sign (−). ND, not determined.