Figure 2.

Dual-choice MBOAT assay. The fly MBOAT proteins Oys, Nes, and Frj were expressed in the acyltransferase-deficient ale1Δ yeast strain. Isolated microsomes containing the fly proteins were incubated with a mixture of eight acyl-CoA species and six lysophospholipids, and the products formed by each enzyme were separated and quantified by LC/MS/MS. Scales are adjusted to highlight the substrate preferences of each enzyme. Results given are the mean ± SEM of three experiments. Acyl-CoAs are abbreviated as x:y, where x is the number of carbon atoms in the chain and y is the number of double bonds.