Figure 3.
Deletion alleles of nes abolish nes expression. (A) Three independent alleles of nes were identified after imprecise excision of the P{EPgy2}nes[EY22898] element. The three predicted transcripts of nes differ only in the first exon, which constitutes the 5′UTR. Untranslated regions are shown in gray, the open reading frame in black, introns as thinner lines, and the deletions in red. All three deletions extend beyond the second exon, which includes the start codon (arrowhead). (B–E) show in situ hybridization with a nes-specific probe. Wild-type blastoderm embryos show strong maternal nes expression (B); nes RNA is incorporated into the germ cells (arrow). Later in embryogenesis, zygotic nes expression is detected ubiquitously (D). This expression is abolished in embryos produced by nesΔ52 mutant parents, stained in parallel (C and E).