Figure 8.

Vps8 recruitment to LE. (A) Localization of Vps8 in the absence of Vps3. BY strains expressing Vps8 from the GAL1-promoter were localized in wild-type and vps3Δ cells with or without GAL1-overproduced Vps21. Cells were analyzed by fluorescence microscopy as described in Figure 1A. Size bar, 5 μm. (B) Localization of Vps8 to LE. SEY6210 strains expressing Vps8 from the TEF1 promoter were localized in wild-type and vps4Δ cells with or without ADH1-overproduced Vps21. Cells were analyzed by fluorescence microscopy as described in Figure 1A. Size bar, 5 μm. Similar results were obtained with BY strains. (C) Model of Vps8, Vps3, and Vps21 recruitment order during the maturation of endosomes. EE, early endosome; MVB, multivesicular body. See the text for details.