Table 1.

Purification of recombinant sunflower acetoacetyl-CoA thiolase

Purification step	Total protein (mg)	Total activity (nkat)	Specific activity (nkat/mg)	Yield (%)	Purification (-fold)
Lysate supernatant	32.4	75	2.2	100	1
10 mM imidazole	0.6	0.0	0.0	0	0
50 mM imidazole	0.069	4.5	66	6.3	29
100 mM imidazole	0.086	22.6	263	31.2	118
125 mM imidazole	0.054	9.1	168	12.5	75
150 mM imidazole	0.0445	4.5	101	6.2	45
250 mM imidazole	0.051	4.1	80	5.6	36
100-250 mM pooled	0.2355	40.2	198	55.5	88