Table 1.

Primer and probe sequences used in real time RT-PCR

gene	forward primer sequence	FW nM	reverse primer sequence	RV nM	Taqman probe sequence	Tqm nM
AVP	TTCCAGAACTGCCCAAGGG	250	AGACACTGTCTCAGCTCCAGGTC	50	SYBR green	---
CRH	TCCCATTTCCCTGGATCTCA	300	GAGCTTGCTGCGCTAACTGA	300	TTCCACCTCCTCCGAGAAGTCTTGGAAAT	250
POMC	CCG GCA ACT GCG ATG AG	900	GGA AAT GGC CCA TGA CGT ACT	900	AGC CGC TGA CTG AGA ACC CCC G	250
oLH	CCGCTCCCAGATATCCTCTTC	150	GTCTGCTGGCTTTGGGAGTTA	150	TCTAAGGATGCCCCACTTCAATCTCCCA	150
oFSH	CCCAACATCCAGAAAGCATGT	900	GCACAGCCAGGCACTTTCA	900	TTCAAGGAGCTGGTGTACGAGACG	150
oPRL	TGAGCTTGATTCTTGGGTTGCT	300	CCCCGCACCTCTGTGACTA	300	CTCCTGGAATGACCCTCTGTATCAC	100
PC1	GCGGGCATCTTCGCTCTAG	900	TCCATACAACCAAGTGCTGCAT	900	AAGCAAATCCAAATCTCACCTGGCGAG	250
PGHS1	GGCACCAACCTCATGTTTGC	100	TCTTGCCGAAGTTTTGAAGA	100	TTCTTTGCCCAACACTTCACCCATCA	200
PGHS2	GCACAAATCTGATGTTTGCATTCT	100	CTGGTCCTCGTTCATATCTGCTT	100	TGCCCAGCACTTCACCCATCAATTTT	200
sgk-1	GACTTTGGACTCTGCAAGGAGAA	900	CGGGCGTGCCACAGAA	900	TTGAACACAATGGCACGACGTCCAC	250