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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Gene Med. 2009 Nov;11(11):1005–1011. doi: 10.1002/jgm.1385

Figure 1.

Figure 1

(A) Crystal violet staining of adenovirus infected cells. Cells were infected with adenoviral vectors in a serial dilution. Ten days after infection, cells were stained with crystal violet to assess comparative cytotoxic effect of AdWT, CRAd-Survivin-5/3, Ad 5/3, def 5/3 or mock in transfected GBM cell lines. (B) Replication of adenoviral vectors. U87MG, U118MG, A172 and No. 10 cell lines were infected with 10 vp/cell of the constructs described above and the cells were harvested at days 1, 3 and 9. Total DNA was isolated from these cells and viral replication was measured by qPCR using primers for the Ad 5 E1A gene. Asterisks (*, **) indicate a statistically significant difference (p < 0.05) at specified time points in gene levels relative to AdWT and Ad 5/3, respectively