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Components used for assembly experiments. (a) Schematic for preparing the DNA construct: the three DNA fragments (dig-sticker, biotin-sticker, and 16.5 kb DNA from pxΔ2 DNA) were prepared separately (see Materials and Methods) and ligated together. (b) Purified histone octamer from chicken erythrocytes and purified recombinant yNap1 were analyzed by 15% SDS-PAGE gel. (c) Analysis of the nucleosome array reconstituted in bulk by 1% agarose gel.
