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. 2009 Dec 16;97(12):3150–3157. doi: 10.1016/j.bpj.2009.09.032

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Real-time assembly of nucleosomal arrays followed as decrease in DNA tether length (Δz) as a result of DNA wrapping around the histone octamer. The flow of histone octamers and Nap1 into the cuvette causes apparent shortening of the tether; once the flow was stopped, the bead went back to its original position. The further changes in Δz reflected array assembly. (a) Example assembly curve on torsionally constrained DNA. The Δz (2.37 μm) indicates partial (∼48%) assembly. Even after 50 min the assembly did not go to completion. (b) Example assembly curve on nicked DNA as control. Inset shows more complete assembly (Δz = 3.20 μm; 78% nucleosome saturation) in ∼4 min.