Figure 2.

Time course of the FokI-catalyzed DNA cleavage reaction. Linearized pSKFok1 DNA (6.3 nM) was incubated at 37°C with 0.2 (A) or 0.6 nM (B) FokI restriction endonuclease in 200 μl of reaction buffer (20 mM Tris-acetate, pH 7.9/10 mM magnesium acetate/50 mM potassium acetate/1 mM DTT/100 μg/ml BSA). At the time points indicated, 10-μl samples were withdrawn, immediately quenched by adding 5 μl of stop solution, and subjected to electrophoresis on 1% agarose-gel.