Figure 7. Activation of Caspases in erythroid cells.

A, Induction of cleaved caspase 3 and cytochrome C in basophilic erythroblasts purified from day 6 cultures 24 hours after incubation with media (controls), anti-CD95 or 6µg/ml hemozoin. B, Western blots of lysates taken from purified basophilic erythroblasts incubated with hemozoin for 4 hours demonstrate increased levels of cleaved caspase 8 and activation of caspase 3 with slight induction of cleaved Bid (tBid). C, Detection of cleaved caspase 9 after 4 and 24 hours incubation with hemozoin. Each band was normalised to that for alpha tubulin. Fold changes in normalized levels of pro-apoptotic proteins relative to those in lysates from media controls are shown below each band.