Figure 5. Immunophenotypic and functional dissection of CX₃CR1⁺ GC B cells.

(A) Double staining of CX₃CR1⁺ GC B cells with CD27 and CD23 mAbs. Each B cell fraction was further characterized with mAbs (CD27⁺ cells, right lower panel; CD23⁺ cells, left lower panel). Results are median percent positive cells, maximum and minimum values from ten different experiments. (B) CX₃CR1⁺ GC B cells were subjected to chemotaxis to 300 ng/ml rCX₃CL1. Migrated cells were double stained for CD27 and CD23. One representative experiment out of seven is shown. (C) Purified GC B cells were subjected to CX₃CL1-driven chemotaxis and migrated cells were collected and stained with a panel of mAbs. Results are median percent positive cells, maximum and minimum values from four different experiments.