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Genomically unstable clone LS-12 shows improper assembly of complex II. Panel A: Western blot showing levels of immunoprecipitated SDHB after transfer on a nitrocellulose membrane. Unstable clone LS-12 shows the presence of SDHB in the soluble fraction. Panel B: Immunoprecipitation of SDHA proteins carried out on 150 μg isolated mitochondria using antibody against SDHA and Sepharose protein A beads. Samples were separated on a 4%–20% gradient gel, which was stained with Coomassie blue.
