Table 1. List of primary antibodies used in this study.
| Antigen | Host | Source, catalog number | Working concentration | Localization |
|---|---|---|---|---|
| M/L cone opsin |
Rabbit polyclonal |
Chemicon, AB5405 |
1:10,000 |
OS of M/L cones |
| S cone opsin |
Rabbit polyclonal |
Chemicon, AB5407 |
1:5,000 |
OS of S cones |
| Bassoom |
Mouse monoclonal |
Abcam, 13249 |
1:200 |
Neuron synapses, OPL and IPL |
| Calbindin |
Rabbit polyclonal |
Swant, CB-38a |
1:1,000 |
Horizontal and amacrine cells |
| Parvalbumin (PV) |
Rabbit polyclonal |
Swant, PV-28 |
1:2,000 |
Amacrine cells |
| Calretinin |
Rabbit polyclonal |
Sigma, C7479 |
1:1,000 |
Amacrine cells and subpopulations of RGCs |
| Calretinin |
Mouse monoclonal |
Swant, CR-39 |
1:1,000 |
Amacrine cells and subpopulations of RGCs |
| Choline acetyltransferase (ChAT) |
Goat polyclonal |
Chemicon AB144P |
1:500 |
Cholinergic amacrine cells and IPL |
| Tyrosine hydroxylase (TH) |
Rabbit polyclonal |
Chemicon, AB152 |
1:500 |
Dopaminergic amacrine cells |
| Goα |
Mouse monoclonal |
Chemicon, MAB3073 |
1:50 |
“on” bipolar cells |
| Protein Kinase C-α (PKC- α) |
Mouse monoclonal |
Santa Cruz, SC-208 |
1:2,000 |
Rod bipolar cells |
| Protein Kinase C-α (PKC- α) |
Rabbit monoclonal |
Santa Cruz, SC-8393 |
1:100 |
Rod bipolar cells |
| Brain Derived Neurotrophic Factor (BDNF) |
Rabbit polyclonal |
Santa Cruz, SC-546 |
1:500 |
Amacrine cells and subpopulations of RGCs |
| Vimentin |
Mouse monoclonal |
Dako, M0725 |
1:2,000 |
Müller cells and astrocytes |
| Glial Fibrillary Acidic Protein(GFAP) | Rabbit polyclonal | Dako, Z0334 | 1:1,000 | Astrocytes |
Detailed information is given about the antibodies used, the host, the commercial source and catalog number, as well as the working concentration and its localization in rat retina. Abbreviations: BDNF represents Brain Derived Neurothrophic factor, CB represents calbindin, ChAT represents Choline acetyltransferase, CR represents Calretinin, GFAP represents glial fibrillary acidic protein, PKC represents protein Kinase C, TH represents tyrosine hydroxilase, IPL represents inner plexiform layer, OPL represents outer plexiform layer, OS represents outer segment, RGCs represents retinal ganglion cells.