Figure 6.

Suppression of E1A-induced apoptosis by mutated p53 in A40 cells is overcome by Ids. Growing cultures of A40 cells (p53−/−) were transfected with either pHβAPr-E1A12S (E1A), pCMV-Id-1H plus pCMV-Id-2H (Id), or a combination of these three expression vectors (E1A + Id) together with pCMVCD20. Control (−), the cells were transfected with pCMVCD20 alone. (A) The expression vectors were transfected in the absence of p53. (B and C) The cells were simultaneously transfected with either pMoLV-wtp53 or pMoLV-p53His175. At the times indicated, the cells were stained with PI, and DNA contents of the cells were analyzed by flow cytometry. The cells were gated based on the expression of CD20.