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Id-2H associates with E1A and E12 bHLH protein in vivo. The cell lysates were prepared from EId10 cells treated with dex for 0, 24, and 48 h, and aliquots of 200 μg of protein were immunoprecipitated with antibodies to E1A, E2A (E12 and E47) and pRB. The precipitates were treated with 0.8% deoxycholate and analyzed by Western blotting with anti-Id2 antibody.
