Figure 1.

Subcellular localization of EB1 in mammalian cells. (a) Specificity of the anti-EB1 mAbs GD10 and EA3 for EB1 in cell lysates (lanes 1 and 3) and immunoprecipitation (lanes 2 and 4). Cell lysates and immunoprecipitated proteins from CV-1 cells, prepared as described, were separated in 10% SDS-polyacrylamide gels, transferred to poly(vinylidene difluoride) membranes, probed with anti-EB1 antibodies (GD10 and EA3), and detected by ECL; heavy chain (HC) and light chain (LC) are indicated. Interphase CV-1 (b and c) and SW480 cells (d) were stained with GD10 antibody after methanol fixation at −20°C. A secondary donkey anti-mouse rhodamine-conjugated antibody was used, and the samples were analyzed by confocal microscopy. [Bars: 25 μm (b) and 10 μm (c and d).]