FIG. 3.

Total amounts and soluble amounts of recombinant proteins in Sf9 cells coinfected at an MOI of 2 with baculoviruses encoding FMDV VP1 (A), human alpha-galactosidase (B), and FMDV VP2 (C) proteins (black bars), compared with protein data obtained during coinfection with a DnaK/DnaJ-encoding baculovirus, also at an MOI of 2 (gray bars). Data were obtained in triplicate at 72 hpi, and they were compared to the total protein amounts observed in the absence of chaperones. Percentages of solubility and VP2 oligomer occurrence are also included. Arrows indicate the positions of the full-length recombinant proteins. The significance of the differences between data values determined in the absence and in the presence of DnaK/DnaJ is indicated through P values from an analysis of variance test.