FIG. 3.

Neighbor-joining tree of S. pistellata-associated bacteria based on 16S rRNA gene sequences. Bootstrapping was performed with 1,000 replications. Selected OTUs from all 16S rRNA gene libraries are presented as S numbers (e.g., S30, a sample isolated in summer), W numbers (e.g., W80, a sample isolated in winter), and SW numbers (e.g., SW7, a sample from both winter and summer). ✶, groups not determined. •, groups detected in both seasons. Reference bacterial sequences selected from the NCBI database were those for Nitrococcus mobilis (L35510), Methylophaga sulfidovorans (X95461), uncultured Thioalkalivibrio sp. (AB189351), Comamonas sp. (AJ002803), uncultured Gammaproteobacteria (AJ969451), Azospirillum lipoferum (Z29619), uncultured Alphaproteobacteria (DQ289899), Hyphomicrobium sp. (AY934488), uncultured Epsilonproteobacteria (AJ515715), uncultured candidate division CAB-I bacterium (DQ200412.1), Synechococcus sp. PCC 7336 (AF448078), Synechococcus sp. WH 7803 (AF311291), Microcystis novacekii (AB035551), uncultured Leptospira sp. (AY995721), Porphyromonas sp. (AF385560), Epulopiscium sp. (M99574), Alkaliphilus metalliredigens (AY137848), Paenibacillus chondroitinus (AB073206), uncultured Brevibacillus sp. (EU647528), Staphylococcus sp. (AJ276810), Bacillus sp. (AF290558), Bacillus benzoevorans (Y14693), Oxalobacteraceae sp. (EU057875), uncultured Deltaproteobacteria (EF076071), uncultured sludge bacterium (AF234754), uncultured Verrucomicrobium (AJ401107), uncultured Gemmata sp. (EU341290), Planctomyces maris (AJ231184), Dietzia sp. (Y17518), and Haloarcula sp. (EU708436).