TABLE 1.
Bacterial strains and plasmids used in this studya
| Strain or plasmid | Relevant description | Source or reference |
|---|---|---|
| Strains | ||
| C. jejuni | ||
| 81-176 | Wild type | Qijing Zhang |
| Δppk1/DG001 | 81-176 derivative with deletion in ppk1 gene; ppk1::Kan | This study |
| Δppk1c/DG002 | DG001 harboring pDG3, Cm | This study |
| 81-176 (dcuA::tetO) | Carries tetracycline resistance marker for natural transformation; Tet | Qijing Zhang |
| E. coli DH5α | Used for cloning | Invitrogen |
| Plasmids | ||
| pZErO-1 | Cloning vector for making suicide vector; Zeo | Invitrogen |
| pRY111 | E. coli-Campylobacter shuttle vector for complementation | 71 |
| pRK2013 | Helper plasmid for conjugation | 1 |
| pUC4K | Source for kanamycin | Amersham |
| pDG1 | pZErO-1 containing ppk1 region plus 1 kb upstream and downstream of 81-176; Zeo | This study |
| pDG2 | A portion of ppk1 replaced with kanamycin resistance region from pUC4K in pDG1; Zeo Kan | This study |
| pDG3 | pRY111 containing ppk1 coding region and the upstream promoter sequence for complementation; Cm | This study |
a
Kan, kanamycin; Cm, chloramphenicol; Zeo, zeocin; tetO, tetracycline resistance gene.