TABLE 1.
Strain or plasmid | Relevant description | Source or reference |
---|---|---|
Strains | ||
C. jejuni | ||
81-176 | Wild type | Qijing Zhang |
Δppk1/DG001 | 81-176 derivative with deletion in ppk1 gene; ppk1::Kan | This study |
Δppk1c/DG002 | DG001 harboring pDG3, Cm | This study |
81-176 (dcuA::tetO) | Carries tetracycline resistance marker for natural transformation; Tet | Qijing Zhang |
E. coli DH5α | Used for cloning | Invitrogen |
Plasmids | ||
pZErO-1 | Cloning vector for making suicide vector; Zeo | Invitrogen |
pRY111 | E. coli-Campylobacter shuttle vector for complementation | 71 |
pRK2013 | Helper plasmid for conjugation | 1 |
pUC4K | Source for kanamycin | Amersham |
pDG1 | pZErO-1 containing ppk1 region plus 1 kb upstream and downstream of 81-176; Zeo | This study |
pDG2 | A portion of ppk1 replaced with kanamycin resistance region from pUC4K in pDG1; Zeo Kan | This study |
pDG3 | pRY111 containing ppk1 coding region and the upstream promoter sequence for complementation; Cm | This study |
Kan, kanamycin; Cm, chloramphenicol; Zeo, zeocin; tetO, tetracycline resistance gene.