TABLE 2.
Primers used in this study
| Name | Sequence (5′-3′)a | Use |
|---|---|---|
| PPK1 F | ATAAAAGGTACCATTTAGCCATAAAACTCCCG | Amplification of 81-176 ppk1 region |
| PPK1 R | AAAAAACTGCAGGCGATAGGTTTGAACCTTTA | |
| PPK1 INV F | ATAAAAGGATCCTACAGATCAAAGTCGTGCAA | Amplification of pDG1 except ppk1 |
| PPK1 INV R | AAAAAAGGATCCGAACATTGGTCTAAAACACG | |
| PPK1 COMP F | AAATAACTGCAGGTCCTAAACCACTTTGCGCT | Amplification of ppk1 gene for complementation |
| PPK1 COMP R | AAATAAGGTACCCCTCGCTTCCACCAGTTTTA | |
| CsrA F | TTATCGGAGAAGGTATAG | Real-time quantitative PCR amplification of cDNA from ppk2, csrA, spoT, phosR, cmeC, pstS, pstC, and CJJ81176_0750 from 81-176 strain |
| CsrA R | TTTCTAAGTATCATAAGGG | |
| SpoT F | GTAACCACTCGCACAATATC | |
| SpoT R | GATGTCGCAGTTTATTCTCC | |
| PhosR F | GCAAACATAATCATCACAACCAC | |
| PhosR R | GAGAGCAAGGATACAAAGAAGC | |
| CmeC F | GCTGCTGCTCAATTAGGTATAG | |
| CmeC R | GCTTCATAATCATACTCACTTGC | |
| PstS F | CCTTATACAAACTGGAATCAAATC | |
| PstS R | GACACATCACTCATTACAAGC | |
| PstC F | CGCTTATGCTTTAGGTATGAC | |
| PstC R | GCTGCCATCACCACTATC | |
| CJJ81176_0750 F | GGTCTTGTTGCCTTATTG | |
| CJJ81176_0750 R | GTATCGCTATGTTCTATGC | |
| PPK2 F | ATCTAATACTCCAACTTGTC | |
| PPK2 R | TTCTTCTTCTCCACTACG |
a
Underlining indicates restriction sites added to the 5′ end of each primer.