TABLE 2.
Name | Sequence (5′-3′)a | Use |
---|---|---|
PPK1 F | ATAAAAGGTACCATTTAGCCATAAAACTCCCG | Amplification of 81-176 ppk1 region |
PPK1 R | AAAAAACTGCAGGCGATAGGTTTGAACCTTTA | |
PPK1 INV F | ATAAAAGGATCCTACAGATCAAAGTCGTGCAA | Amplification of pDG1 except ppk1 |
PPK1 INV R | AAAAAAGGATCCGAACATTGGTCTAAAACACG | |
PPK1 COMP F | AAATAACTGCAGGTCCTAAACCACTTTGCGCT | Amplification of ppk1 gene for complementation |
PPK1 COMP R | AAATAAGGTACCCCTCGCTTCCACCAGTTTTA | |
CsrA F | TTATCGGAGAAGGTATAG | Real-time quantitative PCR amplification of cDNA from ppk2, csrA, spoT, phosR, cmeC, pstS, pstC, and CJJ81176_0750 from 81-176 strain |
CsrA R | TTTCTAAGTATCATAAGGG | |
SpoT F | GTAACCACTCGCACAATATC | |
SpoT R | GATGTCGCAGTTTATTCTCC | |
PhosR F | GCAAACATAATCATCACAACCAC | |
PhosR R | GAGAGCAAGGATACAAAGAAGC | |
CmeC F | GCTGCTGCTCAATTAGGTATAG | |
CmeC R | GCTTCATAATCATACTCACTTGC | |
PstS F | CCTTATACAAACTGGAATCAAATC | |
PstS R | GACACATCACTCATTACAAGC | |
PstC F | CGCTTATGCTTTAGGTATGAC | |
PstC R | GCTGCCATCACCACTATC | |
CJJ81176_0750 F | GGTCTTGTTGCCTTATTG | |
CJJ81176_0750 R | GTATCGCTATGTTCTATGC | |
PPK2 F | ATCTAATACTCCAACTTGTC | |
PPK2 R | TTCTTCTTCTCCACTACG |
Underlining indicates restriction sites added to the 5′ end of each primer.