TABLE 3.

Percentages of viable cells as determined by CTC-DAPI staining^a

Strain	% of viable cells after:
	0 h	0.5 h	1 h	2 h	3 h
Wild type	100	98.4	96.7	94.5	42.4
Δppk1 mutant	100	97.7	30.5	3.5	2.8
Δppk1c mutant	100	98.9	95.2	46.9	27.6

^aA total of 10 fields were counted for each sample, with an average of 90 bacteria in each field. The percentage of viable cells was calculated as follows: % viability = (viable cell count/total cell count) ×100. Total cell counts were determined by adding viable (red) and dead (blue) cell counts. The results represent the mean of three independent experiments.