TABLE 3.
Strain | % of viable cells after: |
||||
---|---|---|---|---|---|
0 h | 0.5 h | 1 h | 2 h | 3 h | |
Wild type | 100 | 98.4 | 96.7 | 94.5 | 42.4 |
Δppk1 mutant | 100 | 97.7 | 30.5 | 3.5 | 2.8 |
Δppk1c mutant | 100 | 98.9 | 95.2 | 46.9 | 27.6 |
A total of 10 fields were counted for each sample, with an average of 90 bacteria in each field. The percentage of viable cells was calculated as follows: % viability = (viable cell count/total cell count) ×100. Total cell counts were determined by adding viable (red) and dead (blue) cell counts. The results represent the mean of three independent experiments.