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RT-PCR analysis of the expression of the monooxygenase genes. PCRs were carried out with primer sets specific for fpdA1 (A) or fpdA2 (B). The carbon sources used for induction were 10 mM glucose (lanes 1 and 2), 1 mM 4-FP (lanes 3 and 4), and 1 mM 4-nitrophenol (lanes 5 and 6). Lanes 1, 3, and 5 were loaded with samples for which the PCR templates were DNase treated but not subjected to the reverse transcriptase reaction (negative control).
