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Kinetic parameters of FpdBa
| Fixed substrateb | Varied substrate | Km (μM) | kcat (s−1) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|
| FAD | NADH | 13 ± 2 | 33 ± 4 | 2.5 × 106 |
| FMN | NADH | 122 ± 14 | 12 ± 2 | 1.0 × 105 |
| NADH | FMN | 10 ± 1 | 63 ± 3 | 6.6 × 106 |
| NADH | FAD | 6 ± 1 | 23 ± 2 | 3.6 × 106 |
Values refer to the varied substrate and are means for triplicate experiments with standard deviations.
FAD and FMN concentrations were fixed at 100 μM, and the NADH concentration was fixed at 300 μM.
