TABLE 3.

Conversion of 4-substituted phenols by purified FpdA2 and FpdB^a

Substrate	Rate (μmol/min mg−1)	Concn (μM)b
		Substrate degraded	Anion formed	Hydroquinone
4-FP	46	344	312	145
4-Chlorophenol	39	292	279	92
4-Bromophenol	72	541	492	98
4-Nitrophenol	40	303	280	150
Hydroquinone	64	130		10c

^aReaction mixtures contained 600 μM substrate, 0.15 mg ml⁻¹ of 4-FP monooxygenase, and the components described in Materials and Methods. Aromatics were analyzed by HPLC or GC at different times, and anions were analyzed by ion chromatography.

^bAfter 20 min of incubation.

^cHydroxyhydroquinone detected by GC.