Fluorescence in situ hybridization was used to visualize Wolbachia distribution in A. albopictus oocytes from the following strains: HT1, aposymbiotic (A); HTMT, tetracycline-cured HTM (B); IH, naturally superinfected strain (C); and HTM, wMelPop-transinfected (D). A high density of Wolbachia is observed in HTM embryos infected with wMelPolp, and the infection is more broadly distributed relative to that observed in naturally infected embryos.