TABLE 2.
Expression of vtpA-lacZ, vtpB-lacZ, vthAB-lacZ, and vtpR-lacZ fusions in E. coli strain Top10 and V. cholerae strain O395N1ΔZ in the presence of hapR or vtpR
| Bacterial construct |
Mean β-galactosidase activity (Miller unit) ± SDa | |
|---|---|---|
| Strain | Plasmids | |
| Top10 | pBAD + pvtpA-lacZ | 91 ± 4 |
| pBAD-hapR + pvtpA-lacZ | 61 ± 8 | |
| pBAD-vtpR + pvtpA-lacZ | 69 ± 18 | |
| O395N1ΔZ | pBAD + pvtpA-lacZ | 209 ± 76 |
| pBAD-hapR + pvtpA-lacZ | 1,670 ± 152 | |
| pBAD-vtpR + pvtpA-lacZ | 1,710 ± 280 | |
| Top10 | pBAD + pvtpB-lacZ | 39 ± 2 |
| pBAD-hapR + pvtpB-lacZ | 1,029 ± 82 | |
| pBAD-vtpR + pvtpB-lacZ | 1,055 ± 124 | |
| O395N1ΔZ | pBAD + pvtpB-lacZ | 120 ± 53 |
| pBAD-hapR + pvtpB-lacZ | 953 ± 89 | |
| pBAD-vtpR + pvtpB-lacZ | 943 ± 130 | |
| Top10 | pBAD + pvthAB-lacZ | 45 ± 15 |
| pBAD-hapR + pvthAB-lacZ | 42 ± 23 | |
| pBAD-vtpR + pvthAB-lacZ | 39 ± 24 | |
| O395N1ΔZ | pBAD + pvthAB-lacZ | 52 ± 24 |
| pBAD-hapR + pvthAB-lacZ | 59 ± 8 | |
| pBAD-vtpR + pvthAB-lacZ | 60 ± 21 | |
| Top10 | pBAD + pvtpR-lacZ | 6,754 ± 1,435 |
| pBAD-hapR + pvtpR-lacZ | 1,764 ± 365 | |
| pBAD-vtpR + pvtpR-lacZ | 1,677 ± 436 | |
| O395N1ΔZ | pBAD + pvtpR-lacZ | 9,820 ± 1,204 |
| pBAD-hapR + pvtpR-lacZ | 2,239 ± 261 | |
| pBAD-vtpR + pvtpR-lacZ | 2,201 ± 497 | |
a
The values are means for three determinations.