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. 2009 Oct 23;37(22):7468–7482. doi: 10.1093/nar/gkp757

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Time course and stability of gene repair. (A) FACS analysis was used to follow GFP protein expression over time. GFP was detected as early as 1 day after ssODN transfection and did not increase significantly after 2 days. Cells transfected with m⁵CpG^GFP showed a significantly greater number of GFP-positive cells as compared with cultures treated with targeting ssODNs, but lacking the methyl-CpG modification. *P ≤ 0.008. (B) FACS dot-plots of myoblasts treated with ssODNs 2 and 7 days after transfection of oligonucleotides. (C) Analysis of cultures by fluorescence microscopy after treatment with the m⁵CpG^GFP ssODN and maintained in culture for 4 days. Bright fluorescence intensity was detected in a subset of replicating cells.

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