Figure 9.

Reduction of MBD4 expression by siRNA interference. (A) RNA interference was used to downregulate expression of MBD4 in myoblast cultures. Cells were transfected with a targeting or control siRNA and gene expression was followed over time by quantitative RT–PCR. Efficient downregulation of MBD4 expression was achieved by 24 h after siRNA transfection and the effect was maintained up to 48 h after transfection. *P ≤ 0.001. (B) Western blot analysis was carried out using total protein isolated 2 days after siRNA transfection. MBD4 protein was markedly reduced in myoblast cultures treated with MBD4 siRNA compared with untreated cells or cells treated with the control siRNA.