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Mechanical unfolding of the HIV-1 hairpin. (A) HIV-1 hairpin sequence and structure. (B) Typical force versus extension curves upon stretching and relaxation of a single molecule using optical tweezers. The relaxation curve has been offset by 50 nm for clarity, since both curves would overlap otherwise. (C) Histogram of contour lengths of unfolded structures. (D) Histogram of unfolding forces.
