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. 2009 Sep 26;37(22):7603–7611. doi: 10.1093/nar/gkp800

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Determination of the rate constants for the exonucleolysis of primer-templates. Data, obtained from gels similar to those shown in Figures 2 and 3, were fitted to a first order decay to show the disappearance of substrate. Only the data obtained with the AA/TT series is shown (similar quality fits were obtained with the GC/CG set). For U+2, a rapid quench apparatus was used; for the other three primer-templates, manual stopping of the reactions was sufficient. The rate constants found are given in Table 2.