Table 1.
The primer-templates used in exonuclease and extension assays
| Designation | Sequence |
|---|---|
| Control | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGTTCGTTCGAACAGAGG |
| Mismatch | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGTCCGTTCGAACAGAGG |
| U-1 | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGUTCGTTCGAACAGAGG |
| U 0 | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGTUCGTTCGAACAGAGG |
| U+1 | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGTTUGTTCGAACAGAGG |
| U+2 | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGTTCUTTCGAACAGAGG |
| U+3 | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGTTCGUTCGAACAGAGG |
| U+4 | Cy5-GGGGATCCTCTAGAGTCGACCTGCAGGGCAA |
| (AA/TT) | CCCCTAGGAGATCTCAGCTGGACGTCCCGTTCGTUCGAACAGAGG |
| Control | Cy5-GGGGATCCTCTAGAGTCGACCTGC |
| (GC/CG) | CCCCTAGGAGATCTCAGCTGGACGACCGTTCGTTCGAACAGAGG |
| Mismatch | Cy5-GGGGATCCTCTAGAGTCGACCTGC |
| (GC/CG) | CCCCTAGGAGATCTCAGCTGGACAACCGTTCGTTCGAACAGAGG |
| U+2 | Cy5-GGGGATCCTCTAGAGTCGACCTGC |
| (GC/CG) | CCCCTAGGAGATCTCAGCTGGACGAUCGTTCGTTCGAACAGAGG |
| U+4 | Cy5-GGGGATCCTCTAGAGTCGACCTGC |
| (GC/CG) | CCCCTAGGAGATCTCAGCTGGACGACCUTTCGTTCGAACAGAGG |
| H+2 | Cy5-GGGGATCCTCTAGAGTCGACCTGC |
| (GC/CG) | CCCCTAGGAGATCTCAGCTGGACGAHCGTTCGTTCGAACAGAGG |
| H+4 | Cy5-GGGGATCCTCTAGAGTCGACCTGC |
| (GC/CG) | CCCCTAGGAGATCTCAGCTGGACGACCHTTCGTTCGAACAGAGG |
The top oligodeoxynucleotide serves as the primer strand (written in 5′–3′ direction) and the bottom as the template (3′–5′ direction). Emboldened and underlining is used to highlight bases that differ from the appropriate control template.