Table 2.
Primer-templatea | Rate constantb (min−1) | Rate (relative to appropriate control) |
---|---|---|
Control (AA/TT) | 0.84 ± 0.06 | 1 |
Mismatch (AA/TT) | 9 ± 2 | 10.7 |
U+2 (AA/TT) | 48 ± 12 | 57 |
U+4 (AA/TT | 0.42 ± 0.06 | 0.5 |
Control (GC/CG) | 0.011 ± 0.001 | 1 |
Mismatch (GC/CG) | 0.061 ± 0.007 | 5.5 |
U+2 (GC/CG) | 1.8 ± 0.3 | 163 |
U+4 (GC/CG) | 0.008 ± 0.0007 | 0.7 |
H+2 (GC/CG) | 1.5 ± 0.3 | 136 |
H+4 (GC/CG) | 0.009 ± 0.004 | 0.8 |
aThe full primer–template sequences are given in Table 1.
bEach rate constant is the average of three values ± SD.