Table 2.

The rate constants, determined under single turnover conditions, for the 3′–5′ proofreading exonuclease of Pfu-Pol with different primer-templates

Primer-templatea	Rate constantb (min−1)	Rate (relative to appropriate control)
Control (AA/TT)	0.84 ± 0.06	1
Mismatch (AA/TT)	9 ± 2	10.7
U+2 (AA/TT)	48 ± 12	57
U+4 (AA/TT	0.42 ± 0.06	0.5
Control (GC/CG)	0.011 ± 0.001	1
Mismatch (GC/CG)	0.061 ± 0.007	5.5
U+2 (GC/CG)	1.8 ± 0.3	163
U+4 (GC/CG)	0.008 ± 0.0007	0.7
H+2 (GC/CG)	1.5 ± 0.3	136
H+4 (GC/CG)	0.009 ± 0.004	0.8

^aThe full primer–template sequences are given in Table 1.

^bEach rate constant is the average of three values ± SD.