Table 3.

The rate constants, determined under single turnover conditions, for the 3′–5′ proofreading exonuclease of Pfu-Pol V93Q with different primer-templates

Primer-templatea	Rate constantb (min−1)	Rate (relative to control)
Control (GC/CG)	0.0026 ± 0.0006	1
U+2 (GC/CG)	0.0067 ± 0.002	2.6
U+4 (GC/CG)	0.0034 ± 0.001	1.3
H+2 (GC/CG)	0.003 ± 0.0003	1.1
H+4 (GC/CG)	0.0027 ± 0.0001	1

^aThe full primer–template sequences are given in Table 1.

^bEach rate constant is the average of three values ± SD.