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. 2009 Oct 21;37(22):7546–7559. doi: 10.1093/nar/gkp828

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Semi-quantitative reverse transcription PCR validation of selected genes identified by microarray analysis as significantly altered by MexT. Total RNA was isolated from PAO1 containing pME6032 (WT vector), PAO1 containing pME6032-mexT (WT mexT) or PAO1ΔmexEF containing pME6032-mexT (ΔEF mexT). The identity of the genes targeted in each set of PCRs is indicated at the bottom of the figure.