Table 3.
The regulatory effect of MexT on the expression of the promoter-lacZ fusions of putative target genes
| Strain (plasmid)→ | PAO1 | PAO1 | PAO1 nfxC | E. coli DH5a | E. coli DH5a | |
|---|---|---|---|---|---|---|
| Promoter-lacZ↓ | (pME6032) | (pME6032-mexT) | PAO1 nfxCΔmexT | (pME6032)a | (pME6032-mexT)a | |
| pMP-PAmexEp | 45 ± 1 | 3991 ± 912 | 954 ± 21 | 33 ± 2 | 9 ± 1 | 230 ± 1 |
| pMP-PA1744p | 120 ± 32 | 2801 ± 143 | NAb | NA | 7 ± 3 | 7520 ± 849 |
| pMP-PA2759p | 52 ± 5 | 896 ± 104 | NA | NA | 2 ± 1 | 4147 ± 666 |
| pMP-PA3229p | 76 ± 19 | 4852 ± 120 | NA | NA | 4 ± 1 | 1148 ± 96 |
| pMP-PA4354p | 824 ± 83 | 3385 ± 462 | NA | NA | 3 ± 1 | 143 ± 1 |
| pMP-PA4623p | 23 ± 3 | 4302 ± 30 | 1714 ± 86 | 29 ± 4 | 41 ± 4 | 6479 ± 724 |
| pMP-PA4881p | 34 ± 11 | 1217 ± 91 | 716 ± 43 | 20 ± 1 | 8 ± 2 | 7783 ± 465 |
| Mutated DNA motif: | ||||||
| pMP-PA4623m1p | 30 ± 10 | 39 ± 6 | 25 ± 2 | 26 ± 1 | 21 ± 19 | 18 ± 12 |
| pMP-PA4881m1p | 38 ± 9 | 12 ± 5 | 22 ± 3 | 20 ± 2 | 13 ± 3 | 12 ± 1 |
| Homologous genes across Pseudomonas species: | ||||||
| pMP-Pfl2659p | NA | NA | NA | NA | 9 ± 1 | 801 ± 15 |
| pMP-Pfl3748p | NA | NA | NA | NA | 7 ± 1 | 10 474 ± 202 |
| pMP-PP3425p | NA | NA | NA | NA | 4 ± 1 | 2231 ± 168 |
| pMP-PP4858p | NA | NA | NA | NA | 2 ± 1 | 3288 ± 43 |
aIPTG (1 mM final conc.) was added in the medium.
bNA, not assayed.