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. 2009 Oct 20;37(22):7407–7415. doi: 10.1093/nar/gkp859

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — In vivo competition assay to inhibit the sns5 insulator function in transgenic embryos. Wild-type histone gene construct H3-H2A-H1 and the deletion mutant ΔpH3-H2A-ΔpH1 (showed in Figure 2A), were microinjected with excess of the BoxA sequence element into S. granularis zygotes. RNase protection was carried out as for Figure 2. The protected 409, 357 and 209 nt RNA bands, respectively, for the H2A, H3 and H1 transcripts are indicated.