. Author manuscript; available in PMC: 2010 Apr 1.

Published in final edited form as: J Solution Chem. 2009 Apr 1;38(4):471–483. doi: 10.1007/s10953-009-9380-9

Table 11.

Values of pH at 25 and 37°C for ACES buffer solutions using data for E_j corrections

Cell B				25°C			37°C
m₁	m₂	m₃	Ionic strength, I	Without^a E_j corr	With^b E_j corr	Calc^c	Without^a E_j corr	With^b E_j corr	Calc^c
0.04	0.08	0.00	0.08	7.041	7.050	7.051	6.836	6.846	6.846
0.08	0.08	0.08	0.16	6.765	6.800	6.801	6.550	6.586	6.587
0.03	0.06	0.10	0.16	7.001	7.037	7.038	6.813	6.850	6.851
0.02	0.04	0.12	0.16	7.066	7.103	7.104	6.801	6.838	6.839

pH = 7.415 + [(E – 0.68275)/ 0.059156] where 7.415 is the physiological phosphate buffer standard at 25°C, and pH = 7.395 + [(E−0.69144) / 0.061538] where 7.395 is the pH of phosphate buffer at 37°C; for example 0.68275 and 0.69147 are the emf values (in volts) from Table 9 at 25 and 37°C, respectively. This is based on the NIST convention for γ_Cl.

Calculation by Eq. (2) where reported liquid junction potentials apply for cells (B and C)

Values obtained from Table 6–Table 8