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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Nature. 2008 Dec 31;457(7227):281–287. doi: 10.1038/nature07682

Figure 1.

Figure 1

IpRGCs of melanopsin-tdTomato transgenic mice. a, Stacked confocal view of ipRGCs (transgenic line 6 (Ln 6); Supplementary Information S1) in flat-mount transgenic retina, showing melanopsin immunoreactivity and tdTomato fluorescence. b, Top panels: live in situ (Line 6) tdTomato cell in flat-mount retina (inner limiting membrane overlying cell removed); bottom panels: live, dissociated tdTomato cell. Infrared-DIC (left) and tdTomato fluorescence (right). c, Whole-cell or perforated-patch recordings of intrinsic photoresponses to dim and bright flashes (at time 0) from in situ wild-type ipRGCs (retrograde-labelled from the suprachiasmatic nucleus) and tdTomato ipRGCs of three transgenic lines (Lines 2, 4 and 6). 50-ms flash. −80 mV holding voltage. d, Responses to current injection recorded from retrograde-labeled wild-type ipRGC, in situ and dissociated tdTomato ipRGCs (Line 6), and conventional RGC retrograde-labelled from optic chiasm. Current monitor below. Steady injected current gave ~ −70 mV resting voltage and stimulus currents adjusted to give similar membrane polarization for all cells. 23°C. Synaptic blockers present for in situ cells. 10-μm scale bars in a and b.